abstract：:Halobacterium halobium contains two gas vacuole protein genes that are located in plasmid pHH1 (p-vac) and in the chromosomal DNA (c-vac). The mutation frequency for these genes is different: the constitutively expressed p-vac gene is mutated with a frequency of 10(-2), while the chromosomal gene expressed in the stat...

journal_title：Canadian journal of microbiology

authors： Pfeifer F,Blaseio U,Horne M

更新日期：1989-01-01 00:00:00

abstract：:Forty different antibiotics with diverse kingdom and functional specificities were used to measure the functional characteristics of the archaebacterial translation apparatus. The resulting inhibitory curves, which are characteristic of the cell-free system analyzed, were transformed into quantitative values that were...

journal_title：Canadian journal of microbiology

authors： Amils R,Ramírez L,Sanz JL,Marín I,Pisabarro AG,Ureña D

更新日期：1989-01-01 00:00:00

abstract：:The origin of the eukaryotic nucleus is difficult to reconstruct. While eukaryotic organelles (chloroplast, mitochondrion) are eubacterial endosymbionts, the source of nuclear genes has been obscured by multiple nucleotide substitutions. Using evolutionary parsimony, a newly developed rate-invariant treeing algorithm,...

journal_title：Canadian journal of microbiology

authors： Lake JA

更新日期：1989-01-01 00:00:00

abstract：:The purpose of this study was to evaluate experimentally the immunogenicity in rabbits of rubella subunits adsorbed to the adjuvant Quil A. The adsorbed viral proteins form structurally defined ImmunoStimulating COMplexes (ISCOMs). Rubella ISCOMs were tested for their capacity to induce neutralizing and hemagglutinati...

journal_title：Canadian journal of microbiology

authors： Trudel M,Nadon F,Séguin C,Payment P

更新日期：1988-12-01 00:00:00

abstract：:The catabolism of glucose by Streptomyces C5, a producer of anthracycline antibiotics, was investigated to determine the pathways that supply precursors for anthracycline biosynthesis. Carbons for the biosynthesis of epsilon-rhodomycinone, an anthracycline aglycone, from radiolabelled glucose were derived primarily fr...

journal_title：Canadian journal of microbiology

authors： Dekleva ML,Strohl WR

更新日期：1988-11-01 00:00:00

abstract：:In this work a bacterial classification method based on the discriminant analysis of the microcalorimetric data provided by the growth power-time (p-t) curves is developed. This method is applied to classify several species of Enterobacteria of different origins, and the results are compared with those obtained by con...

journal_title：Canadian journal of microbiology

authors： Bermúdez J,López D,Sanahuja A,Viñas M,Lorén JG

更新日期：1988-09-01 00:00:00

abstract：:Like recF and recQ mutants studied earlier, two other classes of Escherichia coli mutants defective in the RecF conjugal recombination pathway, recJ and recO, were found to be partially resistant to thymineless death. In contrast, a recN mutant, also belonging to the pathway, was indistinguishable from the wild type w...

journal_title：Canadian journal of microbiology

authors： Nakayama K,Shiota S,Nakayama H

更新日期：1988-07-01 00:00:00

abstract：:High resolution two-dimensional PAGE was used to analyse protein variation among serotype 1 poliovirus isolates. Viruses isolated from patients with recent histories of vaccination with live attenuated poliovirus were compared with prototype serotype 1 poliovaccine. The nonvaccine Mahoney and Brunenders strains of ser...

journal_title：Canadian journal of microbiology

authors： Cash P

更新日期：1988-06-01 00:00:00

abstract：:Addition of Tween 80 to a 1.5% solution of beef extract was found to enhance the elution of bacteriophages adsorbed to electronegative filters. When reconcentration of the eluate was attempted by ammonium sulfate precipitation, a floating layer containing most of the viruses was formed. This floating layer can be obta...

journal_title：Canadian journal of microbiology

authors： Armon R,Arella M,Payment P

更新日期：1988-05-01 00:00:00

abstract：:We examined factors related to the potent antagonistic effect of Escherichia coli and Bacteroides ovatus on Staphylococcus aureus in anaerobic continuous flow cultures. In the presence of sugars fermentable by E. coli alone or both E. coli and S. aureus, motile E. coli strains exerted a potent antagonistic effect and ...

journal_title：Canadian journal of microbiology

authors： Ushijima T,Ozaki Y

更新日期：1988-05-01 00:00:00

abstract：:The deliberate application of the methodology of contemporary molecular genetics to problems in bacterial systematics has led to a broad new understanding of the evolutionary history of both prokaryotes and eukaryotes. In this review, I discuss some of the major conclusions of this endeavour and try to predict future ...

journal_title：Canadian journal of microbiology

authors： Doolittle WF

更新日期：1988-04-01 00:00:00

abstract：:We present preliminary data on the role of antibiotics in intraabdominal sepsis using a new, clinically relevant animal model. Peritoneal cavity infection was induced by ligation and perforation of the cecum in adult rats. Surviving rats were randomized to receive either saline or cefoxitin at the time of cecal excisi...

journal_title：Canadian journal of microbiology

authors： Bohnen JM,Matlow AG,Mustard RA,Christie NA,Kavouris B

更新日期：1988-03-01 00:00:00

abstract：:The 40-kDa porin protein of Haemophilus influenzae type b was reconstituted into proteoliposomes. The relative rates of diffusion of small uncharged sugars across the channels formed by this protein were determined by measuring the rates of osmotic swelling of the liposomes. From these rates, a pore diameter of 1.8 nm...

journal_title：Canadian journal of microbiology

authors： Vachon V,Kristjanson DN,Coulton JW

更新日期：1988-02-01 00:00:00

abstract：:Raw milk samples from bulk tanks in Ontario were analyzed for the presence of Listeria species. The overall incidence of Listeria species in raw milk was 12.4%. Listeria innocua was most frequently isolated and was found in 9.7% (43/445) of the raw milk samples, while L. monocytogenes and L. welshimeri were each found...

journal_title：Canadian journal of microbiology

authors： Farber JM,Sanders GW,Malcolm SA

更新日期：1988-02-01 00:00:00

abstract：:In Neurospora crassa, heat shock results in the induction of 9 to 11 heat shock proteins (HSP), of which HSP80 is the most abundant and the first to be synthesized. The induction of HSP80 was investigated during normal growth (2% sucrose) and under sucrose starvation. Transfer of mycelium to a medium supplemented with...

journal_title：Canadian journal of microbiology

authors： Roychowdhury HS,Kapoor M

更新日期：1988-02-01 00:00:00

abstract：:Of 33 streptococcal cultures belonging to serological group L, all bound human immunoglobulin (Ig) G, fibrinogen, and fibronectin; 32 bound bovine IgG; 31 bound alpha 2-macroglobulin; 5 bound albumin; and none bound either haptoglobin or IgA. The binding sites for IgG could be isolated from the L streptococci by tryps...

journal_title：Canadian journal of microbiology

authors： Lämmler C,Schaufuss P,Frede C,Blobel H

更新日期：1988-01-01 00:00:00

abstract：:Cultures of Streptomyces peucetius often were observed to divert spontaneously from the production of anthracycline antibiotics to the excretion of acidic metabolites, whether grown in a defined medium or a nutrient-rich medium. The predominant excreted acidic metabolites were determined by high-performance liquid chr...

journal_title：Canadian journal of microbiology

authors： Dekleva ML,Strohl WR

更新日期：1987-12-01 00:00:00

abstract：:Using both fluorescent labelled toxin and antibody--secondary antibody techniques, the Bacillus sphaericus toxin was found to bind strongly to susceptible Culex quinquefasciatus cells, but far less strongly to cells of insensitive insects. An insensitive clone of the C. quinquefasciatus cell line was discovered which ...

journal_title：Canadian journal of microbiology

authors： Davidson EW,Shellabarger C,Meyer M,Bieber AL

更新日期：1987-11-01 00:00:00

abstract：:Bacterial communities that can degrade surfactants have been selected from coastal seawaters contaminated by urban sewages. Only the linear fraction of commercial anionic surfactants was quickly degraded, and a residual fraction representing 10% of the initial concentration always remained. The highest concentrations ...

journal_title：Canadian journal of microbiology

authors： Sigoillot JC,Nguyen MH

更新日期：1987-10-01 00:00:00

abstract：:The gene coding for glycoprotein B2 (gB2) of herpes simplex virus type 2 (HSV-2) strain 333 was mapped and its nucleotide sequence determined. Open reading frame analysis deduced a polypeptide consisting of 902 amino acids and having close homology to gB1 of HSV type 1. Several predicted features of gB2 are consistent...

journal_title：Canadian journal of microbiology

authors： Zwaagstra JC,Leung WC

更新日期：1987-10-01 00:00:00

abstract：:The synergistic interactions between Pseudomonas aeruginosa and virulent or attenuated murine cytomegalovirus (MCMV) were compared in vivo. Virulent MCMV challenge at a dose of 5 X 10(5) pfu/mouse intraperitoneally, followed by intranasal superinfection with 5 X 10(6) cfu/mouse of Pseudomonas aeruginosa after 48 h res...

journal_title：Canadian journal of microbiology

authors： Kournikakis B,Babiuk LA

更新日期：1987-10-01 00:00:00

abstract：:Influenza undergoes extensive antigenic variation in nature. These new antigenic variants invariably supplant the preceding antigenic type that then disappears. In apparent contrast to this, two H3N2 strains that were forwarded to the Laboratory Centre for Disease Control, Ottawa, in 1984 from a Canadian public health...

journal_title：Canadian journal of microbiology

authors： Brown EG

更新日期：1987-09-01 00:00:00

abstract：:The range of polysaccharide-degrading enzymes formed by three anaerobic rumen fungi (Neocallimastix patriciarum, Piromonas communis, and an unidentified isolate (F] was monitored following growth on seven mono-, di-, and poly-saccharide carbohydrate substrates. Enzymes capable of degrading a variety of alpha- and beta...

journal_title：Canadian journal of microbiology

authors： Williams AG,Orpin CG

更新日期：1987-05-01 00:00:00

abstract：:Pyrenocine A, a phytotoxin produced by Pyrenochaeta terrestris (Hansen) Gorenz, Walker and Larson, possesses general antibiotic activity against plants, fungi, and bacteria. Effective doses for 50% inhibition (ED50s) are 4 micrograms/mL for onion seedling elongation; 14, 20, 20, and 25 micrograms/mL for the germinatio...

journal_title：Canadian journal of microbiology

authors： Sparace SA,Reeleder RD,Khanizadeh S

更新日期：1987-04-01 00:00:00

abstract：:Urease activity was measured using whole cells of both long (swarming) and short (nonswarming) populations of Proteus mirabilis from casein hydrolysate agar (CHA) and broth (CHB) cultures, and from brain heart infusion broth (BHIB) cultures. Urease is a constitutive enzyme for both long and short cells, but its activi...

journal_title：Canadian journal of microbiology

authors： Jin T,Murray RG

更新日期：1987-04-01 00:00:00

abstract：:Our purpose was to study bacterial antagonism between a limited number of strictly anaerobic strains and Clostridium perfringens in the intestinal tract of gnotobiotic rodents. Gnotobiotic mice harboring a Bacteroides thetaiotaomicron, a Fusobacterium necrogenes, and a Clostridium sp. strain were protected against pat...

journal_title：Canadian journal of microbiology

authors： Yurdusev N,Nicolas JL,Ladire M,Ducluzeau R,Raibaud P

更新日期：1987-03-01 00:00:00

abstract：:Neisseria polysaccharea (LNP 462, NCTC 11858), proposed as a prototype strain constituting a new taxon in the genus Neisseria, produces copious amounts of polysaccharide when grown on agar containing 1-5% sucrose. Plate-grown cells produced an exocellular polysaccharide which was composed of D-glucose, had [alpha]D +2...

journal_title：Canadian journal of microbiology

authors： Riou JY,Guibourdenche M,Perry MB,MacLean LL,Griffith DW

更新日期：1986-12-01 00:00:00

abstract：:Giardia duodenalis is a common intestinal parasite in most parts of the world. In Canada it is associated with both endemic and epidemic infections that are often transmitted by the waterborne route. Although G. duodenalis strains have been isolated from several animals, the role of other mammals in human infection is...

journal_title：Canadian journal of microbiology

authors： Wenman WM,Meuser RU,Wallis PM

更新日期：1986-12-01 00:00:00

abstract：:Four modes of heating endotoxin in plasma and two different times of heating endotoxin in pyrogen-free water were compared. There were no significant differences in standard curves after heating endotoxin in plasma at 100 degrees C for 1 and for 10 min. However, the standard curve after heating for 10 min at 75 degree...

journal_title：Canadian journal of microbiology

authors： Piotrowicz BI,McCartney AC

更新日期：1986-10-01 00:00:00

abstract：:Lysis of mid-log phase cells of the Gram-positive bacterium, Corynebacterium sepedonicum, by a combination of lysozyme treatment and incubation with alkaline sodium dodecyl sulfate at 56 degrees C led to the recovery of a single plasmid. The plasmid was purified in CsCl density gradients, and its molecular weight esti...

journal_title：Canadian journal of microbiology

authors： Clark MC,Lawrence CH

更新日期：1986-08-01 00:00:00

abstract：:The phototrophic purple sulfur bacterium Thiopedia rosea forms multicellular, gas-vacuolate, regular, flat aggregates (platelets, sheets) held together by slime. Platelets found in eutrophic water consisted of slime (85% of the total wet volume) and 16 cells, while the gas-filled vacuole occupied 44% of the volume of ...

journal_title：Canadian journal of microbiology

authors： Scherrer R,Shull VE

更新日期：1986-07-01 00:00:00

abstract：:alpha-Methyl lysine was investigated as a potential inhibitor of lysine transport in Escherichia coli and Bacillus sphaericus. At equimolar concentrations, no inhibition was observed in either organism, but at 10X and 100X the lysine concentration, alpha-methyl lysine caused a 20-50% reduction in the initial rate of l...

journal_title：Canadian journal of microbiology

authors： Regenstreif CA,Kelland JG,Pansare SV,Vederas JC,Pickard MA

更新日期：1986-06-01 00:00:00

abstract：:Administered orally, Quillaja saponin markedly potentiated the humoral immune responses of mice fed inactivated rabies vaccine, and significantly increased their resistance to subsequent intracerebral challenge with live rabies virus. Although mean serum neutralizing antibody titres were generally 8- to 16-fold higher...

journal_title：Canadian journal of microbiology

authors： Maharaj I,Froh KJ,Campbell JB

更新日期：1986-05-01 00:00:00

abstract：:Sera from mice which have been vaccinated with BCG and challenged with old tuberculin contain gamma interferon. These same sera also express antibacterial activity. Using Staphylococcus aureus we demonstrated that its growth was inhibited at dilutions of sera as high as 1:320. A 4% concentration of sera reduced the gr...

journal_title：Canadian journal of microbiology

authors： Moulton RG,Gerner GD

更新日期：1986-05-01 00:00:00

abstract：:Pseudomonas aeruginosa ATCC 9027 grew on 0.5% (v/v) hexadecane as a sole carbon source in a chemically defined medium which required the addition of Fe3+ and Ca2+. There was a variable and extended lag period before an active growth rate was attained. Visible light microscopic evidence revealed that the bacteria did n...

journal_title：Canadian journal of microbiology

authors： Miguez CB,Beveridge TJ,Ingram JM

更新日期：1986-03-01 00:00:00

abstract：:Immunovirological studies suggest that human respiratory syncytial virus may well be composed of five structural proteins as are other members of the Paramyxoviridae family: the two external membrane glycoproteins H (90 000) and Fo (F1, 49 000; F2, 20 000; disulfide linked), the internal membrane protein M (34 000), t...

journal_title：Canadian journal of microbiology

authors： Trudel M,Nadon F,Séguin C,Ghoubril S,Payment P,Trépanier P

更新日期：1986-01-01 00:00:00

abstract：:A disseminated and fatal infection was established in C57BL mice, injected intraperitoneally with either Neisseria meningitidis B,2b or Haemophilus influenzae type b bacteria plus enhancement factors. The effects of mucin, hemoglobin, and iron dextran as enhancement of bacterial infectivity in mice were evaluated indi...

journal_title：Canadian journal of microbiology

authors： Brodeur BR,Tsang PS,Hamel J,Larose Y,Montplaisir S

更新日期：1986-01-01 00:00:00

abstract：:Antibiotic susceptibilities of 382 strains of Campylobacter jejuni isolated in Alberta, Canada, in 1980 and 1981 were determined. Although none were resistant to erythromycin or gentamicin, 5.4 and 22% of strains were resistant to ampicillin in 1980 and 1981, respectively. Tetracycline resistance was noted in 6.8% of ...

journal_title：Canadian journal of microbiology

authors： Taylor DE,Chang N,Garner RS,Sherburne R,Mueller L

更新日期：1986-01-01 00:00:00

abstract：:The site specificity of metallic ion binding in Escherichia coli K-12 lipopolysaccharide was assessed by collecting high-resolution phosphorus nuclear magnetic resonance spectra in the presence of manganese, a paramagnetic divalent cation. This technique revealed high-affinity interactions between the cation and all o...

journal_title：Canadian journal of microbiology

authors： Ferris FG,Beveridge TJ

更新日期：1986-01-01 00:00:00

abstract：:Guanidine-resistant (gr) mutants of poliovirus were previously categorized into four groups by electrophoretic properties and peptide maps of nonstructural virus protein 2C. Growth of mutants in the presence of guanidine depends upon temperature of incubation. The four groups of gr variants respond differently to temp...

journal_title：Canadian journal of microbiology

authors： Tershak DR

更新日期：1985-12-01 00:00:00